Cell Biology Tests

1. Cell proliferation assay

 

 

 

Lanli Biology has more than 1,300 cell lines for pharmacodynamic studies, including more than 600 human tumor cells that have been tested for STR and mycoplasma. There are also nearly 60 mouse cell lines and nearly 600 other cell lines.

 

 

A variety of experimental conditions (such as 96-well plates, 384-well plates, drug treatment duration, etc.), a variety of detection methods (such as CTG, CCK8, MTT, CyQUANT, etc.) are available. Please consult for details.info@lanlybio.com。

 

2. Combined drug administration experiment

In order to help customers better understand the combined effects of drugs, Lanli Biology provides combined drug administration studies based on 96-well and 384-well plates. Fixed-ratio and Matrix-ratio are available, and the experimental results can provide: dose-response curve, CI value (Combination Index value), inhibition heat map of concentration combination, 2D contour map, 3D reaction surface map, etc., which can effectively help you understand the effect of drug combination.

 

 

 

3. Q-PCR, ELISA, WB detection of target genes and proteins

Lanli Biology provides a variety of methods for detecting target genes or proteins.

 

 

4. FACS-based cell cycle and apoptosis analysis

FACS is one of the most commonly used technical means in cell cycle detection. Its basic basis is that the genomic DNA content varies in different stages of the cell cycle. Dyes such as PI are used to specifically bind to nucleic acids, and the fluorescence intensity of PI and other dyes represents the content of nucleic acids.

 

 

Annexin V is used in combination with 7-AAD to detect the early occurrence of apoptosis and distinguish early apoptotic cells from late apoptotic or necrotic cells. When Annexin V-FITC is used in combination with 7-AAD, 7-AAD is excluded from living cells (Annexin V-/7-AAD -) and early apoptotic cells (Annexin V+/7-AAD -), while late apoptotic cells and necrotic cells are stained with FITC and 7-AAD at the same time and show double positive (AnnexinV+/7-AAD +)

 

 

5. Construction of stable cell lines

(1) Construction of stable gene overexpression cell lines

It refers to adding regulatory elements upstream of the target gene through artificial construction, transfecting cells through viral packaging, integrating into the host cell genome, and thus transcribed and translated in large quantities to achieve overexpression of gene products. Gene overexpression can be used to study the biological function of the target gene or obtain a large amount of target protein.

 

 

(2) Construction of gene knockout cell lines

Using CRISPR/Cas9 technology to inactivate or delete specific genes in cell lines can be used to find suitable drug targets.

Using CRISPR/Cas9 technology to knock out the target gene Protein-X in a cell line, Western Blot identifies positive monoclonal cells